Utilization of SDS-PAGE and histochemistry for pharmacognostical studies on selected mangroves and halophytes from the Pichavaram, South India

M. Johnson, G. Adaikala Raj, T. Shibila, P. Ramakrishnan, I. R. A. Menezes, J. G. M. Costa and H. D. M. Coutinho ()
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M. Johnson: St. Xavier’s College (Autonomous)
G. Adaikala Raj: St. Xavier’s College (Autonomous)
T. Shibila: St. Xavier’s College (Autonomous)
P. Ramakrishnan: St. Xavier’s College (Autonomous)
I. R. A. Menezes: Regional University of Cariri – URCA
J. G. M. Costa: Regional University of Cariri – URCA
H. D. M. Coutinho: Regional University of Cariri – URCA

Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, 2020, vol. 22, issue 8, No 21, 7607-7618

Abstract: Abstract This study focused on revealing the pharmacognostical marker, viz. histochemical and protein profiles for Avicennia marina (Forssk.) Vierh., Rhizophora mucronata Lam, Excoecaria agallocha L., Suaeda maritima Dumort and Sesuvium portulacastrum L. The proteins of the studied plants were isolated using phosphate buffer and separated using SDS-PAGE. The separated profiles were visualized using silver staining. The results of the histochemical analysis indicated the profuse presence of polyphenols, tannin and lignin in the epidermis and hypodermis of leaves, stem and roots of selected mangroves and halophytes. Lignin, tannin and polyphenols showed their occurrence in large quantities at endodermis, xylem and phloem of leaves, stem and roots. A total of 40 bands with multiple regions of activity with varied MW–Rf values were observed in the SDS-PAGE system of studied mangrove and halophytes.

Keywords: Pharmacognosy; Mangroves; Halophytes; Histochemistry; Protein profile (search for similar items in EconPapers)
Date: 2020
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DOI: 10.1007/s10668-019-00538-7

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