Gene Expression in a Space-Simulating Magnetically Shielded Environment
Pietro Volpe () and
Tamilla Eremenko
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Pietro Volpe: University of Rome “Tor Vergata”
Tamilla Eremenko: Former Institute of Experimental Medicine, CNR
Environment Systems and Decisions, 2005, vol. 25, issue 2, 83-92
Abstract:
Summary A magnetic-field nullification achieved in a magnetically shielded room or a magnetic field of 70 μT generated in a solenoid with a sinusoidal wave of 50 Hz plus 45 μT DC of Earth, although able to change the cell proliferation and the physico-chemical properties of the cell membrane, did not seem to significantly influence gene expression in Friend erythroleukemia cells. In fact, when the culture growth cycle of these cells underwent magnetic-field deprivation or irradiation, the transcriptional and translational rates, in both undifferentiating and dimethylsulfoxide-differentiating cultures, turned out to be irrelevant. This result was supported by the magnetic-field independent expression of the genes for α and β hemoglobin chains.
Keywords: cell-cycle kinetics; in vitro differentiation; tRNA; rRNA and polyA-containing pre-mRNA transcripts; nascent protein chains; hemoglobin; subtraction and enrichment of the geomagnetic field (search for similar items in EconPapers)
Date: 2005
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DOI: 10.1007/s10669-005-4270-1
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