EconPapers    
Economics at your fingertips  
 

High-affinity ouabain receptors: primary membrane sensors for ionizing radiation

A. I. Dvoretsky, A. M. Shainskaya and S. N. Ayrapetyan ()
Additional contact information
A. I. Dvoretsky: Oles Honchar Dnepropetrovsk National University
A. M. Shainskaya: Weizmann Institute in Rehovot
S. N. Ayrapetyan: UNESCO Chair-Life Sciences International Postgraduate Educational Center

Environment Systems and Decisions, 2012, vol. 32, issue 2, 242-248

Abstract: Abstract Although the high sensitivity of the Na/K pump in cell membrane to ionizing radiation is well known in literature, the individual role of different isoforms of pump in determination of its radio-sensitivity is not clear yet. This is the subject of the present investigation. Using isotope, electro-physiological and enzymological methods, the effect of γ-ionizing radiation on cell membrane voltage-current characteristics, acetylcholine-induced membrane current, 22Na+ and 45Ca2+ exchange between cells and bathing solution, Na+K+-ATPase activity, dose-dependent ouabain binding with cell membrane, intracellular cAMP and membrane phosphorylation in snail neurons were studied. The changes in neurons as a result of 30-min γ-radiation exposure of snails to 5.16 Ci/kg at the end of the first 30 min of post-radiation period were as follows: the increase in membrane ionic conductance reversed the ouabain sensitivity of acetylcholine-induced currents, stimulation of 22Na+ and 45Ca2+ uptakes, inhibition of Na/K pump, activation Na/Ca exchange in reversed mode, increase in ouabain binding with high-affinity α3 and decrease with α2 middle-affinity receptors, decrease in intracellular cAMP content and membrane dephosphorylation. On the basis of the obtained data, it is suggested that both α3 and α2 catalytic subunits of Na++K+-ATPase serve primary membrane sensors through the activation of which the biological effect of γ-radiation on neurons is realized. The IR has activation effects on α3-dependent Na+/Ca2+ exchange in forward and its inactivation on α2-dependent reverse modes.

Keywords: γ-radiation; Neuron; Na/K pump; Na+/Ca2+ exchange; Isoform; Receptor (search for similar items in EconPapers)
Date: 2012
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://link.springer.com/10.1007/s10669-012-9393-6 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:spr:envsyd:v:32:y:2012:i:2:d:10.1007_s10669-012-9393-6

Ordering information: This journal article can be ordered from
https://www.springer.com/journal/10669

DOI: 10.1007/s10669-012-9393-6

Access Statistics for this article

More articles in Environment Systems and Decisions from Springer
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().

 
Page updated 2025-03-20
Handle: RePEc:spr:envsyd:v:32:y:2012:i:2:d:10.1007_s10669-012-9393-6