An automated bacterial colony counting and classification system

Wei-Bang Chen () and Chengcui Zhang ()
Additional contact information
Wei-Bang Chen: University of Alabama at Birmingham
Chengcui Zhang: University of Alabama at Birmingham

Information Systems Frontiers, 2009, vol. 11, issue 4, No 2, 349-368

Abstract: Abstract Bacterial colony enumeration is an essential tool for many widely used biomedical assays. However, bacterial colony enumerating is a low throughput, time consuming and labor intensive process since there may exist hundreds or thousands of colonies on a Petri dish, and the counting process is usually manually performed by well-trained technicians. In this paper, we introduce a fully automatic yet cost-effective bacterial colony counter which can not only count but also classify colonies. Our proposed method can recognize chromatic and achromatic images and thus can deal with both color and clear medium. In addition, the proposed method is software-centered and can accept general digital camera images as its input. The counting process includes detecting dish/plate regions, identifying colonies, separating aggregated colonies, and reporting colony counts. In order to differentiate colonies of different species, the proposed counter adopts one-class Support Vector Machine (SVM) with Radial Basis Function (RBF) as the classifier. Our proposed counter demonstrates a promising performance in terms of both precision and recall, and is robust and efficient in terms of labor-and time-savings.

Keywords: Biomedical image mining; Bacterial colony counting; Segmentation; Clustering; Classification; One-class SVM (search for similar items in EconPapers)
Date: 2009
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
http://link.springer.com/10.1007/s10796-009-9149-0 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:spr:infosf:v:11:y:2009:i:4:d:10.1007_s10796-009-9149-0

Ordering information: This journal article can be ordered from
http://www.springer.com/journal/10796

DOI: 10.1007/s10796-009-9149-0

Access Statistics for this article

Information Systems Frontiers is currently edited by Ram Ramesh and Raghav Rao

More articles in Information Systems Frontiers from Springer
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().