Application of FTIR-ATR Spectroscopy for Determination of Glucose in Hydrolysates of Selected Starches

Bartošová Alica (), Soldán Maroš, Sirotiak Maroš, Blinová Lenka and Michaliková Anna
Additional contact information
Bartošová Alica: Institute of Safety and Environmental Engineering, Department of Environmental Engineering, Faculty of Materials Science and Technology, Slovak University of Technology, Botanická 49, 917 24 Trnava, Slovak Republic
Soldán Maroš: Institute of Safety and Environmental Engineering, Department of Environmental Engineering, Faculty of Materials Science and Technology, Slovak University of Technology, Botanická 49, 917 24 Trnava, Slovak Republic
Sirotiak Maroš: Institute of Safety and Environmental Engineering, Department of Environmental Engineering, Faculty of Materials Science and Technology, Slovak University of Technology, Botanická 49, 917 24 Trnava, Slovak Republic
Blinová Lenka: Institute of Safety and Environmental Engineering, Department of Environmental Engineering, Faculty of Materials Science and Technology, Slovak University of Technology, Botanická 49, 917 24 Trnava, Slovak Republic
Michaliková Anna: Institute of Safety and Environmental Engineering, Department of Environmental Engineering, Faculty of Materials Science and Technology, Slovak University of Technology, Botanická 49, 917 24 Trnava, Slovak Republic

Research Papers Faculty of Materials Science and Technology Slovak University of Technology, 2013, vol. 21, issue Special-Issue, 116-121

Abstract: Fourier transform infrared spectroscopy was evaluated as an easy and simple analytical method for determination of starch residues after enzymatic hydrolysis. Different starch sources were liquefaction by α-amylase enzyme Termamyl SC for 25 minutes in autoclave. In the next step were starches solutions enzymatically hydrolysed by enzyme pollulanase Promozyme® for 24 hours to 60°C water bath. Total glucose in starch hydrolysate was determined using Fourier Transformed Infrared Spectroscopy (FTIR) with ATR accessory with diamante crystal by recording the absorption of different carbohydrate in spectral range from 700 - 4000 cm-1. Based on calibration curves of glucose the release of total glucose in hydrolysates was calculated.

Keywords: starch; enzymatic hydrolyse; sugars; FTIR-ATR (search for similar items in EconPapers)
Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://doi.org/10.2478/rput-2013-0019 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:vrs:repfms:v:21:y:2013:i:special_issue:p:116-121:n:19

DOI: 10.2478/rput-2013-0019

Access Statistics for this article

Research Papers Faculty of Materials Science and Technology Slovak University of Technology is currently edited by Kvetoslava Rešetová

More articles in Research Papers Faculty of Materials Science and Technology Slovak University of Technology from Sciendo
Bibliographic data for series maintained by Peter Golla ().