INVESTIGATION ON THE MORPHOLOGY OF PRECIPITATED CHEMICALS FROM TE BUFFER ON SOLID SUBSTRATES

Huabin Wang, Lijuan Zhang, Feng Zhang, Hongjie An, Shimou Chen, Hai Li, Peng Wang, Xinyang Wang, Yang Wang and Haijun Yang
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Huabin Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Lijuan Zhang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Feng Zhang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China
Hongjie An: Tianjin University of Science and Technology, Tianjin 300040, P. R. China
Shimou Chen: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Hai Li: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Peng Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Xinyang Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Yang Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Haijun Yang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;

Surface Review and Letters (SRL), 2007, vol. 14, issue 06, 1121-1128

Abstract: Buffer is often involved in the biological studies on a surface with the scanning probe microscopy and the remnant of the salts of buffer may affect the correct interpretation of the experimental results. However, the knowledge on the performance of chemicals of buffer on a substrate is still very poor. TE solution (Tris–HCl,NaCl, and ethylene diamine tetraacetic acid (EDTA)) is a widely used buffer in stocking biological molecules. Herein, we report the performance of the precipitated chemicals from TE solution on two typical substrates with several commonly used sample preparation methods. The results showed that the chemicals in TE solution could self-organize into parallel nanofilaments on hydrophobic highly oriented pyrolitic graphite (HOPG) surface by blotting the shortly incubated solution droplet from the substrate or by drying the diluted solution droplet naturally. In contrast, no such special structures were observed on hydrophilic mica using the same methods. By imaging in TE solutions, no special structures were found on either the HOPG or the mica. Moreover, the effects of the concentration of chemicals of TE buffer on the morphology of precipitated chemicals were also investigated.

Keywords: Buffer; morphology; highly oriented pyrolitic graphite (HOPG); mica; atomic force microscopy (search for similar items in EconPapers)
Date: 2007
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DOI: 10.1142/S0218625X0701069X

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