DISPERSION AND FIXATION OF ADENO-ASSOCIATED VIRUS WITH GLUTARALDEHYDE FOR AFM STUDIES

Peng Wang, Xinyan Wang, Haijun Yang and Junhong Lü ()
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Peng Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Xinyan Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Haijun Yang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Junhong Lü: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Surface Review and Letters (SRL), 2008, vol. 15, issue 05, 595-598

Abstract: Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%,v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.

Keywords: Adeno-associated virus (AAV); atomic force microscope (AFM); glutaraldehyde; dispersion; fixation (search for similar items in EconPapers)
Date: 2008
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DOI: 10.1142/S0218625X08011688

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