SIZE AND SURFACE EFFECT OF GOLD NANOPARTICLES(AuNPs)IN NANOGOLD-ASSISTED PCR

Jiehuan Huang, Xiaodong Zhang (), Chunmei Wang, Lihua Wang, Haikuo Li, Xueyan Cao, Aili Zhang, Xueling Li, Chunhai Fan and Jun Hu
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Jiehuan Huang: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Xiaodong Zhang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P.O. Box 800-204, Shanghai 201800, P. R. China
Chunmei Wang: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Lihua Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P.O. Box 800-204, Shanghai 201800, P. R. China
Haikuo Li: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Xueyan Cao: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Aili Zhang: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Xueling Li: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Chunhai Fan: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China;
Jun Hu: College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China;

Surface Review and Letters (SRL), 2008, vol. 15, issue 06, 757-762

Abstract: Recently, gold nanoparticles(AuNPs)were reported to increase the specificity and efficiency of the polymerase chain reaction (PCR). In this paper, we tested the enhancement ofAuNPswith five different sizes on the specificity of two-round PCR. The results showed that, except 5.02 nmAuNPs, theAuNPsthat could achieve the similar enhancement happened to have nearly the same total surface area. The surface effect seems to be the key factor of nanogold-assisted PCR.

Keywords: PCR; gold nanoparticles; size effect (search for similar items in EconPapers)
Date: 2008
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DOI: 10.1142/S0218625X08012189

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