VISUALIZATIONEX SITUOF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS

Xinyan Wang (), Haijun Yang, Huabin Wang, Peng Wang and Hai Li
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Xinyan Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Haijun Yang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
Huabin Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China
Peng Wang: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China
Hai Li: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Surface Review and Letters (SRL), 2009, vol. 16, issue 01, 79-85

Abstract: Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNAin vitrotests using DNase I incubation based on high-resolution AFM imagingex situwith fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation and enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension and strong immobilization on a surface and discover the mechanochemistry.

Keywords: Visualization; atomic force microscopy; ex situ; modified dynamic molecular combing; DNase I; incubation; degradation (search for similar items in EconPapers)
Date: 2009
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DOI: 10.1142/S0218625X09012329

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