QUARTZ CRYSTAL MICROBALANCE STUDY OF KINETICS AND THERMODYNAMICS OF IgG ADSORPTION ON THE POLYSTYRENE SURFACE

Bo Feng (), Xi Feng, Xiaomei Xie, Caiyun Wang, Mengxue Zhang and Xiaoyun Zhang
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Bo Feng: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, P. R. China
Xi Feng: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, P. R. China
Xiaomei Xie: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, P. R. China
Caiyun Wang: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, P. R. China
Mengxue Zhang: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, P. R. China
Xiaoyun Zhang: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, P. R. China

Surface Review and Letters (SRL), 2015, vol. 22, issue 02, 1-8

Abstract: Polystyrene (PS) is a common substrate material for protein adsorption on biosensors and bioassays. By employing a quartz crystal microbalance (QCM) technique, we studied the kinetics and thermodynamics of anti-HBeAg adsorption on this substratein situ. First, a thin PS film was deposited on the interface between the liquid and the quartz crystal wafer. Second, a solution containing anti-HBeAg was introduced into the holding tank. Third, we measured the change in the oscillation frequency of the quartz that was induced by the antibody–PS binding, which in turn depended on macroscopic parameters, such as antibody concentration and solution temperature, as well as on microscopic parameters, such as the diffusion type and the change in Gibb's free energy. The adsorption rate and capacity of anti-HBeAg on the PS surface were quantified and fitted using the conventional Langmuir model. It was shown that this model was applicable to protein–PS interface system. Approximately 250 nm2of the surface area was occupied by a single antibody molecule at 298 K when the concentration was 90 μg/mL. The adsorption process might have been controlled by both liquid film and particle diffusions.

Keywords: Quartz crystal microbalance; polystyrene; IgG antibody; adsorption kinetics and thermodynamic (search for similar items in EconPapers)
Date: 2015
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DOI: 10.1142/S0218625X15500262

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