A GRAPHENE/ENZYME-BASED ELECTROCHEMICAL SENSOR FOR SENSITIVE DETECTION OF ORGANOPHOSPHORUS PESTICIDES

Nong Zhou, Chengyong Li (), Rijian Mo, Peng Zhang, Lei He, Fanghong Nie, Weiming Su, Shucheng Liu, Jing Gao, Haiyan Shao, Zhong-Ji Qian and Hongwu Ji
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Nong Zhou: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Chengyong Li: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Rijian Mo: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Peng Zhang: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Lei He: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Fanghong Nie: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Weiming Su: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Shucheng Liu: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Jing Gao: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Haiyan Shao: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China
Zhong-Ji Qian: #x2020;Department of Chemistry, Pukyong National University, Busan 608737, Korea
Hongwu Ji: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China

Surface Review and Letters (SRL), 2016, vol. 23, issue 02, 1-8

Abstract: A sensitive and fast sensor for quantitative detection of organophosphorus pesticides (OPs) is obtained using acetylcholinesterase (AChE) biosensor based on graphene oxide (GO)–chitosan (CS) composite film. This new biosensor is prepared via depositing GO–CS composite film on glassy carbon electrode (GCE) and then assembling AChE on the composite film. The GO–CS composite film shows an excellent biocompatibility with AChE and enhances immobilization efficiency of AChE. GO homogeneously disperses in the GO–CS composite films and exhibits excellent electrocatalytic activity to thiocholine oxidation, which is from acetylthiocholine catalyzed by AChE. The results show that the inhibition of carbaryl/trichlorfon on AChE activity is proportional to the concentration of carbaryl/trichlorfon. The detection of linear range for carbaryl is from 10nM to 100nM and the correlation coefficients of 0.993. The detection limit for carbaryl is calculated to be about 2.5nM. In addition, the detection of linear range for trichlorfon is from 10nM to 60nM and the correlation coefficients of 0.994. The detection limit for trichlorfon is calculated to be about 1.2nM. This biosensor provides a new promising tool for trace organophosphorus pesticide detection.

Keywords: Graphene oxide; Chitosan; carbaryl; trichlorfon; biosensor (search for similar items in EconPapers)
Date: 2016
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DOI: 10.1142/S0218625X15501036

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