COMPARISON OF THE IN VITRO CYTOTOXICITIES OF NITROGEN DOPED (p-TYPE) AND n-TYPE ZINC OXIDE NANOPARTICLES

Junko Fujihara, Hideki Hashimoto (), Naoki Nishimoto (), Miki Tongu () and Yasuhisa Fujita ()
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Junko Fujihara: Department of Legal Medicine, Shimane University Faculty of Medicine, 89-1 Enya, Izumo, Shimane 693-8501, Japan
Hideki Hashimoto: Department of Biomedical Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan
Naoki Nishimoto: Project for Advanced Technology Innovation, Shimane Institute for Industrial Technology, 1 Hokuryo, Matsue, Shimane 690-0816, Japan
Miki Tongu: Department of Pharmacy, Shin-Yamanote Hospital, Japan Anti-Tuberculosis Association, 3-6-1 Suwa, Higashimurayama, Tokyo 189-0021, Japan
Yasuhisa Fujita: Interdisciplinary Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu Matsue, Shimane 690-8504, Japan

Surface Review and Letters (SRL), 2018, vol. 25, issue 04, 1-8

Abstract: The use of NPs in the health care field is increasing. Before their biological application, investigating the toxicities of both n-type ZnO nanoparticles (NPs) and nitrogen-doped (“p-type”) NPs is important. Using L929 cells, the cell viability, oxidative stress, apoptosis induction, inflammatory responses, and cellular uptake were assayed 24h after the addition of n-type ZnO NPs and nitrogen-doped NPs (which act as p-type) (25μg/mL). The ZnO NPs were fabricated using a gas evaporation method. Increased H2O2 generation and decreased levels of glutathione were more evident in with n-type than in those treated with nitrogen-doped (“p-type”) ZnO NPs. Caspase-3/-7 activity was higher in cells treated with n-type ZnO NPs than in those treated with nitrogen-doped (“p-type”) NPs. Elevated levels of TNF-α and IL-1β were observed in cell culture supernatants: IL-1β levels were higher in n-type ZnO NPs than nitrogen-doped (“p-type”) NPs. The cellular Zn uptake of n-type ZnO NPs was higher than nitrogen-doped (“p-type”) NPs. These findings show that n-type ZnO NPs have higher cytotoxicity than nitrogen-doped (“p-type”) ZnO NPs. This may be due to a reductive effect of n-type ZnO NPs that induces higher free radical production, reactive oxygen species (ROS) generation, and cellular uptake of this type of ZnO NPs.

Keywords: ZnO; NPs; nitrogen-doped; n-type; toxicity; L929 cells; ROS (search for similar items in EconPapers)
Date: 2018
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DOI: 10.1142/S0218625X18500841

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