Lactic Acid Bacteria With Antimicrobial Properties Isolated From The Instestines Of Japanese Quail (Coturnix Coturnix Japonica)

Nurul Amni Zaidi, Azzmer Azzar and Tengku Haziyamin Tengku Abdul Hamid
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Nurul Amni Zaidi: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang
Azzmer Azzar: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang
Tengku Haziyamin Tengku Abdul Hamid: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang

Science Heritage Journal (GWS), 2017, vol. 1, issue 1, 10-12

Abstract: Lactic acid bacteria (LAB) confer many advantages to humans and animals and they provide natural protection against bacterial pathogens such as Salmonella typhymurium and Eschericia coli. LAB strains have been isolated from various animal sources but from this research LAB were isolated from quail’s (Coturnix coturnix Japonica) intestine. About 12 strains were isolated from the quail’s intestine; and out of these, 5 samples (S1-1B, S2-1A, S2-1B, S2-2B and S3-1A) produced lactose on lactose test. All of these strains appeared to be non-motile coccus with gram positive morphology, catalase-negative and oxidase-negative. These strains showed antimicrobial activities (inhibition zones) against indicator bacterium, i.e., Staphylococcus aureus, Escherichia coli and Salmonella typhimurium using agar well methods. PCR amplification of 16S ribosomal RNA revealed that these five isolates show highest sequence similarity with other LAB strains isolated from other animals which include common genera such as Enterococcus and Lactobacillus. Strain S1-1B, S2-1B and S2-2B were highly similar with Enterococcus faecium, strain S3-2B with Enterococcus durans and S2-1A with Lactobacillus salivarius. This work shows that common LAB species were isolated from intestinal tissues of Malaysian quail (Coturnix Japonica) which were able to produce antimicrobial activities against pathogenic strains.

Keywords: Lactic acid bacteria; quail intestine; probiotics; enterococcus (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:zib:zbngws:v:1:y:2017:i:1:p:10-12

DOI: 10.26480/gws.01.2017.10.12

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