Isolation And Identification Of Halophilic Bacteria Producing Halotolerant Protease

Nadiah Syuhada Abd Samad, Azura Amid, Dzun Noraini Jimat and Nurul Aqilah Ab. Shukor
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Nadiah Syuhada Abd Samad: Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia.
Azura Amid: Bioprocess and Molecular Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia
Dzun Noraini Jimat: Bioprocess and Molecular Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia
Nurul Aqilah Ab. Shukor: Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia.

Science Heritage Journal (GWS), 2017, vol. 1, issue 1, 7-9

Abstract: Halotolerant proteases are known as one of the important groups of enzymes that have been used widely in various industries. However, high production cost of proteases in term of energy used for sterilization and high risk of microbial contamination during fermentation become the obstacles during upstream processing. Moreover, proteases that capable to withstand with harsh conditions of salinity, temperature and pH are required in industry. Therefore, this study presents newly isolated halophilic bacteria producing halotolerant proteases. Halophilic bacteria were isolated from fermented fish sauce. Screening of the bacteria producing halotolerant protease enzymes was carried out by using skim milk salt agar containing 10% NaCl at pH 7 and incubated under aerobic condition at 37oC for 2 days. The selected isolates were identified based on their morphology followed by 16S rDNA gene sequence analysis. 40 colonies of halophiles bacteria were isolated, however, only 20 of them showing proteolytic activity. All of 20 isolates are motile and gram positive bacteria. From 20 isolates, only 6 were chosen for further analysis. B7 showed the highest proteolytic activity compared with others. Results of 16S rDNA gene sequence analysis showed 98% homology to Bacillus amyloliquefaciens subsp. plantarum strain FZB42. Therefore, B7 is identified as Bacillus amyloliquefaciens strain B7.

Keywords: Halotolerant Protease; Halophiles Bacteria; Bacteria Identification (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:zib:zbngws:v:1:y:2017:i:1:p:7-9

DOI: 10.26480/gws.01.2017.07.09

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