The Effect Of Aqueous Extracts Of Nigella Sativa On Breast Cancer Cell Line Mcf-7: An In Vitro Study

Muhammad Solehuddin Bumidin, Farah Azurin Johari, Nurul Fikri Risan and Mohd Hamzah Mohd Nasir ()
Additional contact information
Muhammad Solehuddin Bumidin: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
Farah Azurin Johari: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
Nurul Fikri Risan: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
Mohd Hamzah Mohd Nasir: Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia

Science Heritage Journal (GWS), 2018, vol. 2, issue 1, 13-17

Abstract: Nigella sativa (NS) or also known as black seeds is one of the traditional herbs that were identified to have anticancer properties due to the presence of an active compound, thymoquinone. In this study, the cytotoxic effect of the aqueous extracts of NS were tested on the breast cancer cell line, MCF-7. Aqueous extraction of NS using cold water producing 3.8% of yield. The cytotoxic effect of aqueous extracts of NS were tested after incubation at 5 hours, 24 hours and 48 hours by using a modified MTT colorimetric assay. Interestingly, the IC50 of aqueous extracts of NS on MCF-7 was at 11.5 µg/ml. Additionally, aqueous extracts of NS at the concentration beyond 11.5 µg/ml causing the swelling of MCF-7 and leads to cell burst. In conclusion, aqueous extracts of NS have been proved to have cytotoxic effect on MCF-7.

Keywords: Nigella sativa; aqueous extracts; MCF-7; cytotoxic (search for similar items in EconPapers)
Date: 2018
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://jscienceheritage.com/download/787/ (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:zib:zbngws:v:2:y:2018:i:1:p:13-17

DOI: 10.26480/gws.01.2018.13.17

Access Statistics for this article

Science Heritage Journal (GWS) is currently edited by Professor Dr. Ahmed Jalal Khan Chowdhury

More articles in Science Heritage Journal (GWS) from Zibeline International Publishing
Bibliographic data for series maintained by Zibeline International Publishing ( this e-mail address is bad, please contact ).