A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot and mouth disease in District Faisalabad, Pakistan during the Year 2016

Waqas Ali, Mudasser Habib, Sanaullah Sajid, Rai Shafqat Ali Khan, M. Usman Mazhar, Irfan Ullah Khan, Uneeb Saliha, Muhammad Farooq, Irfan Ullah Khan, Uneeb Saliha, Muhammad Farooq, M. Salah Ud Din Shah and Hafiz Muhammad Muzammil
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Waqas Ali: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Mudasser Habib: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Sanaullah Sajid: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Rai Shafqat Ali Khan: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
M. Usman Mazhar: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Irfan Ullah Khan: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Uneeb Saliha: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Muhammad Farooq: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Irfan Ullah Khan: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Uneeb Saliha: Institute of Microbiology, Faculty of veterinary science, University of Agriculture, Faisalabad, Pakistan
Muhammad Farooq: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
M. Salah Ud Din Shah: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
Hafiz Muhammad Muzammil: Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan

Matrix Science Medica (MSM), 2017, vol. 1, issue 1, 27-29

Abstract: Foot and mouth disease is an economically devastating disease of livestock that mainly effect cloven-hoofed animals i.e. sheep, goat, cattle, pig, buffalo, deer etc. The aim of this study was to determine the serotypes circulating in the region during 2016. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the confirmation of FMDV genome. Out of total 72 samples, 65 were found positive which were then serotyped into type O (n=30), Asia1 (n=19) and A (n=5). Some samples (n=5) were found positive for more than one serotype that were subjected to reverse transcriptase loop-mediated isothermal amplification assay (RT-LAMP) for serotype determination.

Keywords: FMD; reverse transcriptase loop-mediated isothermal amplification assay (RT-LAMP); type O; type Asia1; type A (search for similar items in EconPapers)
Date: 2017
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:zib:zbnmsm:v:1:y:2017:i:1:p:27-29

DOI: 10.26480/msm.01.2017.27.29

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