Laboratory-Scale Biodegradation of Fuel Oil No. 6 in Contaminated Soils by Autochthonous Bacteria

Hilda Amelia Pinon-Castillo, Daniel Lardizabal-Gutierrez, Francisco Javier Zavala-Diaz De La Serna, Daniel Hernandez-Castillo, Laila N. Munoz-Castellanos, Blanca E. Rivera-Chavira and Guadalupe Virginia Nevarez-Moorillon

A chapter in Advances in Bioremediation and Phytoremediation from IntechOpen

Abstract: In order to evaluate the degradation of fuel oil no. 6 (FO6) in contaminated soil, laboratory-scale bioreactors were set up to study biostimulation, bioaugmentation, and natural attenuation processes. A solution of fertilizers was added in biostimulation and biouagmentation (0.03% N, 0.01% P). To the bioaugmentation process, an enrichment culture of indigenous hydrocarbon-degrading microorganisms was also added once a week. Total aerobic and hydrocarbon-degrading microorganisms were determined by plate count, and total petroleum hydrocarbon (TPH) concentration was determined gravimetrically (EPA method 9071b) every 15 days. After 1 year of study, degradation rate was higher for biostimulation (0.19 g TPH/day), followed by natural attenuation (0.18 g TPH/day) and bioaugmentation (0.16 g TPH/day). TPH showed a change in composition of hydrocarbons, attributed to microbiological activity. Microbial counts of hydrocarbon-degrading microorganisms were on the range of 4-6 log CFU/g soil. Preliminary bacterial identification corresponded to Pseudomonas, Rhodococcus, Actinomyces, and Bacillus strains; randomly amplified polymorphic DNA (RAPD); and denaturing gradient gel electrophoresis (DGGE) analysis demonstrated a large microbial diversity. From the degradation rates, it can be predicted that such limits will be achieved by increasing further 107-117 days of the treatments. Results demonstrated to be efficient on the restoration of contaminated soil, being an alternative to treat soils contaminated with heavy hydrocarbons.

Keywords: fuel oil no. 6; biostimulation; bioaugmentation; natural attenuation; PCR-DGGE (search for similar items in EconPapers)
JEL-codes: Q57 (search for similar items in EconPapers)
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Persistent link: https://EconPapers.repec.org/RePEc:ito:pchaps:120507

DOI: 10.5772/intechopen.70350

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