Development of an Ethanol Biosensor Based on Silver Nanoparticles/Polyaniline/Graphite/Epoxy Composite for Friendly Analytical Application

Eliana Mosse Alhadeff, Alfredo Jackson Telles Bosco, Caio Fragale Pastusiak, Thais Anjos Correia and Ninoska Isabel Bojorge Ramirez

A chapter in Biosensors for Environmental Monitoring from IntechOpen

Abstract: Electrochemical biosensors for measuring ethanol were developed in this study. Silver nanoparticles were incorporated to increase sensibility. Firstly, the transducer element was prepared with silver nanoparticles/polyaniline/graphite/epoxy composite (AgNPs/PANI/GEC) where the enzymes alcohol oxidase (AOD) and horseradish peroxidase (HRP) were immobilized by adsorption. The composition of the immobilized solution was indicated by an experimental design (85% of the bi-enzymatic solution, 10% of albumin and 5% of glutaraldehyde). The immobilization method adopted in this study showed to be highly reproducible. The values of variance and standard deviation were low (0.003 and 0.053, respectively--means of three electrodes). The linear range was 0-30 g/L (R2 = 0.983) and the sensitivity 0.004 mA L/g. A second biosensor was made with a transducer prepared with AgNPs/PANI/GEC. A chitosan film was applied over the adsorbed enzymatic solution to avoid desorption of the immobilized enzymes. The AgNPs/PANI/GEC electrodes were characterized using cycle voltammetry and the composite surface by scanning electron microscopy. The calibration for ethanol samples with this second composite fitted in a range of 0.0-0.35 g/L (R2 = 0.984). Square rate voltammetry was the electrochemical analytical method used to obtain the amperometric calibration curves.

Keywords: alcohol oxidase; horseradish peroxidase; polyaniline; silver nanoparticles; electrochemical biosensor (search for similar items in EconPapers)
JEL-codes: L65 (search for similar items in EconPapers)
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Persistent link: https://EconPapers.repec.org/RePEc:ito:pchaps:179014

DOI: 10.5772/intechopen.89359

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