Combination Therapy of Green Tea and Green Coffee on Improving Cardiomyocyte Metabolism Through Increased Expression of AMPK and AKT Genes in Metabolic Syndrome Model Rats

Indah Nur Chomsy (), Mohammad Saifur Rohman, Husnul Khotimah, Nashi Widodo and Nur Ida Panca Nugrahini
Additional contact information
Indah Nur Chomsy: University of Brawijaya, Doctoral Program of Medical Science, Faculty of Medicine
Mohammad Saifur Rohman: University of Brawijaya, Department of Cardiology and Vascular Medicine, Saiful Anwar General Hospital, Faculty of Medicine
Husnul Khotimah: University of Brawijaya, Laboratory of Pharmacology, Faculty of Medicine
Nashi Widodo: Brawijaya University, Biology Department, Faculty of Mathematics and Natural Sciences
Nur Ida Panca Nugrahini: University of Brawijaya, Doctoral Program of Food Sciences, Department of Food Science and Biotechnology, Faculty of Agricultural Technology

A chapter in Proceedings of the 2022 Brawijaya International Conference (BIC 2022), 2023, pp 477-487 from Springer

Abstract: Abstract Metabolic syndrome (MetS) is a set of risk factors for metabolic abnormalities that can lead to dysfunction of organs, one of which is the heart. Each risk factor component of MetS represents a significant economic burden for patients, health plans, and society at large. One of the cardiovascular diseases (CVD) found is decrease in heart contractility. This cardiac dysfunction begins with abnormal expression of several proteins involved in lipid and glucose metabolism pathways, such as AMP-activated protein kinase (AMPK) and Protein Kinase B (Akt). One of the efforts to increase the use of environmentally friendly and sustainable resources, the use of herbs is the main focus in various treatments, one of which is MetS. This study aims to determine the effect of combination therapy of green tea and green coffee with metformin on cell metabolism pathways through the expression of AMPK and AKT genes in the heart of MetS model rats. Rats model were treated with metformin (MFN) 100 mg/kgbw, and/or green tea and green coffee extract (GTCE and/or COMB) 300 mg/kgbw and 200 mg/kgbw respectively. Rat hearts were isolated and analyzed by reverse-transcriptase PCR method using AMPK and AKT primers. The results showed that there was a significant difference in AMPK gene expression (p-value = 0.000) and AKT (p-value = 0.002) between all groups. The correlation between the two shows that they have a correlation with each other. This study showed that the combination therapy of green tea and green coffee with metformin was able to improve cell metabolic pathways by increasing the expression of AMPK and AKT genes in the heart of MetS model rats.

Keywords: AMP-activated protein kinase; Protein Kinase B; Metabolic syndrome; metformin; green tea and coffee extract (search for similar items in EconPapers)
Date: 2023
References: Add references at CitEc
Citations:

There are no downloads for this item, see the EconPapers FAQ for hints about obtaining it.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:spr:advbcp:978-94-6463-140-1_48

Ordering information: This item can be ordered from
http://www.springer.com/9789464631401

DOI: 10.2991/978-94-6463-140-1_48

Access Statistics for this chapter

More chapters in Advances in Economics, Business and Management Research from Springer
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().