Photochemical Scheme for Reversibly Photoswitchable Fluorescent Proteins Used in Biological Imaging

A. Pellissier-Tanon (), R. Chouket, T. Le Saux, L. Jullien, A. Espagne and A. Lemarchand ()
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A. Pellissier-Tanon: PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure
R. Chouket: PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure
T. Le Saux: PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure
L. Jullien: PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure
A. Espagne: PSL University, Sorbonne Université, CNRS, Pasteur, Département de chimie, École normale supérieure
A. Lemarchand: Laboratoire de Physique Théorique de la Matière Condensée, Sorbonne Université, CNRS

A chapter in Trends in Biomathematics: Stability and Oscillations in Environmental, Social, and Biological Models, 2022, pp 23-36 from Springer

Abstract: Abstract The complex kinetics of reversibly photoswitchable fluorescent proteins (RSFPs) is exploited in biological imaging. We analyze the fluorescence evolution of RSFPs submitted to two-color illumination at different light intensities appropriately chosen to change the rate-limiting steps of photoswitching. Starting from a reasonable seven-state photoswitching mechanism, we use the steady-state approximation to obtain simple reduced mechanisms associated with a small number of characteristic times. The comparison with experimental results for the RSFP Dronpa-2 provides previously unknown kinetic information on photoswitching.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:spr:sprchp:978-3-031-12515-7_2

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DOI: 10.1007/978-3-031-12515-7_2

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