Comparison of extraction methods in vitro Plasmodium falciparum: A1H NMR and LC-MS joined approach

Lúcia Mamede, Fanta Fall, Matthieu Schoumacher, Allison Ledoux, Céline Bugli, Bernadette Govaerts and E.A.,
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Lúcia Mamede: Université de Liège
Fanta Fall: Université catholique de Louvain
Matthieu Schoumacher: Université de Liège
Allison Ledoux: Université de Liège
Céline Bugli: Université catholique de Louvain, LIDAM/ISBA, Belgium
Bernadette Govaerts: Université catholique de Louvain, LIDAM/ISBA, Belgium

No 2024006, LIDAM Reprints ISBA from Université catholique de Louvain, Institute of Statistics, Biostatistics and Actuarial Sciences (ISBA)

Abstract: Malaria is a parasitic disease that remains a global concern and the subject of many studies. Metabolomics has emerged as an approach to better comprehend complex pathogens and discover possible drug targets, thus giving new insights that can aid in the development of antimalarial therapies. However, there is no standardized method to extract metabolites from in vitro Plasmodium falciparum intraerythrocytic parasites, the stage that causes malaria. Additionally, most methods are developed with either LC-MS or NMR analysis in mind, and have rarely been evaluated with both tools. In this work, three extraction methods frequently found in the literature were reproduced and samples were analyzed through both LC-MS and 1 H NMR, and evaluated in order to reveal which is the most repeatable and consistent through an array of different tools, including chemometrics, peak detection and annotation. The most reliable method in this study proved to be a double extraction with methanol and methanol/water (80:20, v/v). Metabolomic studies in the field should move towards standardization of methodologies and the use of both LC-MS and 1 H NMR in order to make data more comparable between studies and facilitate the achievement of biologically interpretable information.

Keywords: Metabolomics; Malaria; Plasmodium sp.; Mass spectrometry; Nuclear magnetic resonance; Methodology (search for similar items in EconPapers)
Pages: 9
Date: 2024-02-13
Note: In: Biochemical and Biophysical Research Communications, 2024, vol. 703, 149684
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Persistent link: https://EconPapers.repec.org/RePEc:aiz:louvar:2024006

DOI: 10.1016/j.bbrc.2024.149684

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