A role for plant microtubules in the formation of transmission-specific inclusion bodies of Cauliflower mosaic virus

Alexandre Martiniere (), Daniel Gargani, Marilyne Uzest (), Stéphane Blanc (), Nicole Lautredou () and Martin Drucker ()
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Alexandre Martiniere: BPMP - Biochimie et Physiologie Moléculaire des Plantes - INRA - Institut National de la Recherche Agronomique - Montpellier SupAgro - Centre international d'études supérieures en sciences agronomiques - UM - Université de Montpellier - CNRS - Centre National de la Recherche Scientifique - Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier
Daniel Gargani: UMR BGPI - Biologie et Génétique des Interactions Plante-Parasite - Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement - INRA - Institut National de la Recherche Agronomique - Montpellier SupAgro - Centre international d'études supérieures en sciences agronomiques - Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier
Marilyne Uzest: UMR BGPI - Biologie et Génétique des Interactions Plante-Parasite - Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement - INRA - Institut National de la Recherche Agronomique - Montpellier SupAgro - Centre international d'études supérieures en sciences agronomiques - Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier
Stéphane Blanc: UMR BGPI - Biologie et Génétique des Interactions Plante-Parasite - Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement - INRA - Institut National de la Recherche Agronomique - Montpellier SupAgro - Centre international d'études supérieures en sciences agronomiques - Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier, LPC - Laboratoire de Pathologie Comparée - INRA - Institut National de la Recherche Agronomique - UM2 - Université Montpellier 2 - Sciences et Techniques - CNRS - Centre National de la Recherche Scientifique
Nicole Lautredou: IGH - Institut de génétique humaine - UM - Université de Montpellier - CNRS - Centre National de la Recherche Scientifique
Martin Drucker: LPC - Laboratoire de Pathologie Comparée - INRA - Institut National de la Recherche Agronomique - UM2 - Université Montpellier 2 - Sciences et Techniques - CNRS - Centre National de la Recherche Scientifique

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Abstract: Interactions between microtubules and viruses play important roles in viral infection. The best-characterized examples involve transport of animal viruses by microtubules to the nucleus or other intracellular destinations. In plant viruses, most work to date has focused on interaction between viral movement proteins and the cytoskeleton, which is thought to be involved in viral cell-to-cell spread. We show here, in Cauliflower mosaic virus (CaMV)-infected plant cells, that viral electron-lucent inclusion bodies (ELIBs), whose only known function is vector transmission, require intact microtubules for their efficient formation. The kinetics of the formation of CaMV-related inclusion bodies in transfected protoplasts showed that ELIBs represent newly emerging structures, appearing at late stages of the intracellular viral life cycle. Viral proteins P2 and P3 are first produced in multiple electron-dense inclusion bodies, and are later specifically exported to transiently co-localize with microtubules, before concentrating in a single, massive ELIB in each infected cell. Treatments with cytoskeleton-affecting drugs suggested that P2 and P3 might be actively transported on microtubules, by as yet unknown motors. In addition to providing information on the intracellular life cycle of CaMV, our results show that specific interactions between host cell and virus may be dedicated to a later role in vector transmission. More generally, they indicate a new unexpected function for plant cell microtubules in the virus life cycle, demonstrating that microtubules act not only on immediate intracellular or intra-host phenomena, but also on processes ultimately controlling inter-host transmission.

Keywords: CAULIFLOWER MOSAIC VIRUS; FLUORESCENCE IMAGING; PLANT VIRUS; MICROTUBULES; CELL-MICROBE INTERACTION; VECTOR TRANSMISSION; BIOLOGIE CELLULAIRE; VIRUS DE LA MOSAIQUE DU CHOU-FLEUR; TRANSMISSION DU VIRUS (search for similar items in EconPapers)
Date: 2009-04
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Published in The Plant Journal, 2009, 58 (1), pp.135-146. ⟨10.1111/j.1365-313X.2008.03768.x⟩

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Persistent link: https://EconPapers.repec.org/RePEc:hal:journl:hal-02088280

DOI: 10.1111/j.1365-313X.2008.03768.x

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