Tools for resistance monitoring in oriental fruit moth (Lepidoptera: Tortricidae) and first assessment in Brazilian populations

Myriam Siegwart (), L.B. Monteiro, Sandrine Maugin, Jérôme Olivares, S. Malfitano Carvalho and Benoit Sauphanor
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Myriam Siegwart: PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles - INRA - Institut National de la Recherche Agronomique
L.B. Monteiro: UFPR - Universidade Federal do Paraná [Curitiba, Brasil] = Federal University of Paraná [Curitiba, Brazil] = Université fédérale du Paraná [Curitiba, Brésil]
Sandrine Maugin: PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles - INRA - Institut National de la Recherche Agronomique
Jérôme Olivares: PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles - INRA - Institut National de la Recherche Agronomique
S. Malfitano Carvalho: Departamento de Entomologia - UFLA - Universidade Federal de Lavras = Federal University of Lavras
Benoit Sauphanor: PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles - INRA - Institut National de la Recherche Agronomique

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Abstract: In southern Brazilian apple (Malus spp.) orchards, predominantly organophosphates are used to control the oriental fruit moth, Cydia molesta (Busck) (Lepidoptera: Tortricidae), but control failures often occur. Therefore the susceptibility of three C. molesta Brazilian populations was investigated to five insecticides of different groups and modes of action, in comparison with a susceptible laboratory strain mass reared in southern France for >10 yr. At the same time, comparative biochemical and genetic analysis were performed, assessing the activities of the detoxification enzymatic systems and sequencing a gene of insecticide molecular target to find out markers associated with resistance. The three Brazilian populations were significantly resistant to chlorpyrifos ethyl compared with the reference strain. One of the field populations that had been frequently exposed to deltamethrin treatments showed significant decreasing susceptibility to this compound, whereas none of the three populations had loss of susceptibility to tebufenozide and thiacloprid compared with the reference strain. All three populations had slight but significant increases of glutathione transferase and carboxylesterases activities and significant decrease of specific acetylcholinesterase activities compared with the reference. Only the most resistant population to chlorpyriphos exhibited a significantly higher mixed function oxidase activity than the reference. The acetylcholinesterase of females was significantly less inhibited by carbaryl in the Brazilian populations than in the reference strain (1.7-2.5-fold), and this difference was not expressed in the male moth. However, no mutation in the MACE locus was detected. These biological and molecular characterizations of adaptive response to insecticides in C. molesta provide tools for early detection of insecticide resistance in field populations of this pest

Date: 2011
Note: View the original document on HAL open archive server: https://hal.inrae.fr/hal-02651786v1
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Published in Journal of Economic Entomology, 2011, 104 (2), pp.636-645. ⟨10.1603/EC10302⟩

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Persistent link: https://EconPapers.repec.org/RePEc:hal:journl:hal-02651786

DOI: 10.1603/EC10302

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