Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)

Marcela A. Rodriguez, Dolors Bosch, Benoit Sauphanor and Jesús Avilla
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Marcela A. Rodriguez: Universitat de Lleida, IRTA - Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology
Dolors Bosch: IRTA - Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology
Benoit Sauphanor: PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles - INRA - Institut National de la Recherche Agronomique
Jesús Avilla: Universitat de Lleida, IRTA - Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology

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Abstract: The mechanisms associated with resistance of codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), to organophosphate insecticides in pome fruit orchards have been shown to depend on the area. Our objectives were to evaluate the susceptibility of Spanish codling moth populations to chlorpyrifos-ethyl, azinphos-methyl, and phosalone, and the activity of three enzymatic systems reported to be involved in resistance. Eleven field populations and a susceptible strain used as a reference were tested using a bioassay consisting in the topical application of a diagnostic concentration on postdiapausing larvae. The enzymatic activity of mixed function oxidases (MFO), glutathione transferases (GST), and esterases (EST) was measured in postdiapausing larvae and adults. A significant decrease in the efficacy of the organophosphates was observed for all field populations, although the decrease was smaller in the case of chlorpyrifos-ethyl. No differences between sexes were detected. In postchapausing larvae, the activity of the three enzymatic systems was higher in all the field populations than in the susceptible population. The possible implication of EST in codling moth insecticide resistance is reported for the first time in European field populations. In adults, only MFO and GST were implicated. Codling moth resistance to organophosphates in Spanish populations must be taken into account in the implementation of antiresistance strategies.

Keywords: CYDIA POMONELLA; BIOASSAY; DETOXIFYING ENZYMES; ORGANOPHOSPHATES; RESITANCE DETECTION; LEPIDOPTERA; TORTRICIDAE; INSECTE (search for similar items in EconPapers)
Date: 2010
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Published in Journal of Economic Entomology, 2010, 103 (2), pp.482-491. ⟨10.1603/EC09249⟩

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Persistent link: https://EconPapers.repec.org/RePEc:hal:journl:hal-02658079

DOI: 10.1603/EC09249

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