Development of a Transient Viral CRISPR Expression System to Manipulate Flowering Time in Plants

Citra Recha Sari, Jemma Taylor, Yiguo Hong and Stephen Jackson
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Citra Recha Sari: School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom
Jemma Taylor: School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom
Yiguo Hong: School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom
Stephen Jackson: School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom

International Journal of Applied and Physical Sciences, 2016, vol. 2, issue 3, 65-70

Abstract: Around 180 genes have been found in Arabidopsis that control flowering time based on analysis of transgenic plants [1]. Most of these genes take part in the six main pathways controlling flowering; the photoperiod, vernalization and the ambient temperature pathways that respond to environmental conditions, while the age-dependent, autonomous, and gibberellin pathways involve a response to endogenous signals [2]. Flowering Locus T (FT) is a major gene involved in flowering in plants because FT encodes mobile florigen for floral induction. Mutation of the FT gene can result in delayed flowering. Using a transient CRISPR-Cas9 expression system mediated by virus expression, it is possible to do gene-editing in plants without incorporation of exogenous DNA. We aim to develop these approaches to manipulate flowering time in plants

Keywords: Flowering; FT; CRISPR-Cas9; PVX; Viral Expression (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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Persistent link: https://EconPapers.repec.org/RePEc:apa:ijapss:2016:p:65-70

DOI: 10.20469/ijaps.2.50002-3.pdf

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