Optimization of Xylanase Production from Fermentation of Water Hyacinth (Eichhornia crassipes) using Trichoderma species

Chinyere Blessing Udeh, Joseph Baba Ameh, Saleh Alhaji Ado and Stanley Irobekhian Reuben Okoduwa
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Chinyere Blessing Udeh: Department of Microbiology, Ahmadu Bello University Zaria Nigeria
Joseph Baba Ameh: Department of Microbiology, Ahmadu Bello University Zaria Nigeria
Saleh Alhaji Ado: Department of Microbiology, Ahmadu Bello University Zaria Nigeria
Stanley Irobekhian Reuben Okoduwa: Directorate of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria Nigeria

Journal of Biotechnology Research, 2017, vol. 3, issue 3, 15-24

Abstract: Department of Microbiology, Ahmadu Bello University Zaria Nigeria. Background: In the present study, optimized cultural conditions for enhanced production of xylanase from local soil isolate of Trichoderma species, using water hyacinth as a substrate in submerged culture fermentation is presented. Method: The Megazyme assay method was used for endo 1, 4-?-xylanase using Azo-xylan (Birchwood). Results: A continuous increase in xylanase production was observed with increasing level of substrate concentration in the medium and highest production was obtained with water hyacinth at 6% w/v level. Maximum xylanase production was achieved with a pH 5.0, incubation temperature of 30ºC and agitation rate of 150 rpm. The highest production was achieved on day five of fermentation at optimum parameters under study. Result: The study showed that production of xylanase can be cost effective using water hyacinth and can be implored on large scale for industrial applications.

Keywords: Xylanase; Extracellular enzymes; Microorganism; Environment. (search for similar items in EconPapers)
Date: 2017
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Citations: View citations in EconPapers (1)

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