Synchronized nutrient stress conditions trigger the diversion of CDP-DG pathway of phospholipids synthesis towards de novo TAG synthesis in oleaginous yeast escalating biodiesel production

Alok Patel, Vikas Pruthi and Parul A. Pruthi

Energy, 2017, vol. 139, issue C, 962-974

Abstract: In this study oleaginous yeast, Rhodosporidium kratochvilovae HIMPA1 grown in glucose synthetic medium containing different nitrogen (1 g/l and 0.1 g/l) and phosphorus (0.05 g/l and 0.1 g/l) limited conditions. Among various N and P-limited conditions, the highest lipid content (60.34± 0.69%) obtained under synchronized limitation of N and P (0.1 g/l N and 0.05 g/l P). Live fluorescent cell imaging of yeast cells after BODIPY505-515 nm staining endorses the results of triacylglycerol (TAG) accumulation in lipid droplets. The cells grown in synchronized limitation of N and P exhibited boosted cell size (6.76 ± 0.39 μm) and lipid droplet size (5.62 ± 0.28 μm). Under synchronized limitation of N and P, supersized irregular shaped lipid droplets (LD) coalesced to form big lobules in the cellular compartment of oleaginous yeast having 87.14% enhanced TAG accumulation as depicted by TLC. Synchronized nutrient limitation diverts the CDP-DG pathway of phospholipids synthesis towards de novo TAG synthesis. The maximum increment of oleic acid (C18:1) was reported in synchronized limitation of N and P that improve the biodiesel properties like oxidative stability, viscosity, cetane number and cold filter plugging point.

Keywords: Oleaginous yeast; Lipid accumulation; Fatty acid methyl esters; Biodiesel properties (search for similar items in EconPapers)
Date: 2017
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:energy:v:139:y:2017:i:c:p:962-974

DOI: 10.1016/j.energy.2017.08.052

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