Biomass conversion through optimization of cellulase from Chryseobacterium junjuense Bp17 and their utility in bioethanol production

Narendra Kumar H K, Chandra Mohana N, Amrutha H C, Rakshith D, Harini B P and S. Satish

Energy, 2023, vol. 283, issue C

Abstract: The search for economically viable and less laborous strategy for bioethanol production has increased globally in this quest bacterial cellulase treated biomass has been promising. Potential bacterium isolated from the forest soil near Bandipur of Karnataka, India exploiting its cellulolytic activity on the CMC agar medium. Molecular identification and characterization of isolate Bp17 revealed as Chryseobacterium junjuense Bp17. An optimization study, enhance the yield of the enzyme through varying parameters such as time, temperature, and pH. Enzymatic assays like β 1, 4 endoglucanase (148.55 U/ml), β 1, 4 exoglucanase (37.21 U/ml), and β glucosidase (42.21 U/ml) exhibit the enzyme activity. Endo, exo and β-glucosidase activities of hydrolyzed biomass broth like coconut husk; 212.88 ± 1.24 U/ml, 151.65 ± 1.24 U/ml and 176.63 ± 1.24 U/ml, rice bran; 223.72 ± 1.42 U/ml, 112.88 ± 1.42 U/ml and 133.43 ± 1.42 U/ml, Pongamia leaves; 389.69 ± 2.54 U/ml, 205.92 ± 2.54 and 187.99 ± 2.54 U/ml, and wood waste; 196.86 ± 0.2 U/ml, 138.90 ± 0.2 U/ml and 168.84 ± 0.2 U/ml, respectively. Hydrolyzed and control biomass differences analyzed through Scanning electron microscope (SEM), Energy dispersive X-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. Zymography reveals the activity and molecular weight of cellulolytic enzymes (40-130 KB). Hydrolyzed broth filtrate produced from biomass used as crude enzyme and further used for bioethanol production through the yeast fermentation process.

Keywords: Chryseobacterium junjuense Bp17; SEM-EDX; FT-IR; Substrate gel electrophoresis; And bioethanol (search for similar items in EconPapers)
Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0360544223025811
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:energy:v:283:y:2023:i:c:s0360544223025811

DOI: 10.1016/j.energy.2023.129187

Access Statistics for this article

Energy is currently edited by Henrik Lund and Mark J. Kaiser

More articles in Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().