Efficacy and functional mechanisms of a novel combinatorial pretreatment approach based on deep eutectic solvent and ultrasonic waves for bioconversion of sugarcane bagasse

Vishal Sharma, Parushi Nargotra, Surbhi Sharma and Bijender Kumar Bajaj

Renewable Energy, 2021, vol. 163, issue C, 1910-1922

Abstract: The current study encompasses the development of a combined pretreatment strategy using deep eutectic solvent (DES) i.e. choline chloride (ChCl)/glycerol and ultrasonic waves for the digestibility of sugarcane bagasse (SCB). Pretreated biomass was saccharified by an enzyme cocktail produced in house from a novel fungus Aspergillus assiutensis VS34. Combined ChCl/glycerol and ultrasound pretreatment yielded higher sugars (276.8 mg/g biomass) than either of the individual pretreatments i.e. ChCl/glycerol (235.3 mg/g biomass) or ultrasound (174.5 mg/g biomass). Optimization of pretreatment process variables (biomass loading, sonication amplitude and pretreatment time) by Design of Experiment enhanced sugar yield by 1.12-fold. Sugar hydrolysate obtained was fermented by dual yeast cultures i.e. Saccharomyces cerevisiae and Pichia stipitis to get an ethanol yield of 142.09 mg/g biomass. To understand the molecular mechanism of combined DES/ultrasound pretreatment which led to eased hydrolysis, and enhanced sugar yield, the pretreated biomass was studied by SEM, FT-IR, XRD and 1H NMR analysis for various structural aberrations that made the biomass more amenable to enzymatic hydrolysis. Insights of underlying functional mechanisms of pretreatment may help in developing new/novel designer pretreatment approaches for proficient biomass conversion.

Keywords: Deep eutectic solvent pretreatment; Ultrasound; Sugarcane bagasse; Ethanol; Enzymatic saccharification; Optimization (search for similar items in EconPapers)
Date: 2021
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Citations: View citations in EconPapers (3)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:163:y:2021:i:c:p:1910-1922

DOI: 10.1016/j.renene.2020.10.101

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