Microbial pretreatment using Kosakonia oryziphila IH3 to enhance biogas production and hydrocarbon depletion from petroleum refinery sludge

Shinjini Paul Choudhury, Sugato Panda, Izharul Haq and Ajay S. Kalamdhad

Renewable Energy, 2022, vol. 194, issue C, 1192-1203

Abstract: Petroleum refinery sludge (PRS) from the biological treatment unit was assessed for the extent of anaerobic biodegradability and pollutant removal through microbial pretreatment utilizing a novel lignin peroxidase (LiP) enzyme-producing bacterial strain, Kosakonia oryziphila to optimize the accelerated solubilization of PRS. K. oryziphila was incubated at different dosages (107, 108 and 109 CFU/mL) for 6 d resulting in maximum soluble chemical oxygen demand (3-fold increment) and volatile fatty acids concentration (2.53-fold increment) against untreated for dosage 108 CFU/mL on 4th d. The maximum LiP activity (20.52 IU/mL) was obtained at optimum conditions. The interactive effect of pretreated substrate and inoculum (S/I) ratios and pH on accumulated biogas yield was optimized using central composite design-response surface methodology in 1 L batch assay. This resulted in significant interaction of independent variables to obtain an optimal condition (S/I = 2.69 and pH = 7.38) for maximum biogas (5.15 L/g VSadded) with 1.56-fold increment against untreated PRS. During process scale-up (20 L study), total petroleum hydrocarbon, oil and grease, and total phenol removals were 56.2%, 62.5% and 88.4% respectively within 50 d of digestion. The digestate phytotoxicity assay revealed a significant reduction in seed-germination inhibition with an increase in seedling and biomass growth suggesting improved decontamination during the digestion process.

Keywords: Biogas; Microbial pretreatment; Petroleum refinery sludge; Phytotoxicity; Kosakonia oryziphila (search for similar items in EconPapers)
Date: 2022
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Citations: View citations in EconPapers (2)

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Persistent link: https://EconPapers.repec.org/RePEc:eee:renene:v:194:y:2022:i:c:p:1192-1203

DOI: 10.1016/j.renene.2022.05.167

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