Bio Ethanol Production from Rice Straw Saccharification via Avicelase Gene in E. coli Recombinant Strain

Mohamed S. Abdel-Salam (), Safa S. Hafez, Mohamed Fadel, Shereen A. H. Mohamed, Wafaa K. Hegazy and Bigad E. Khalil
Additional contact information
Mohamed S. Abdel-Salam: Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza 12622, Egypt
Safa S. Hafez: Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11566, Egypt
Mohamed Fadel: Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Giza 12622, Egypt
Shereen A. H. Mohamed: Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza 12622, Egypt
Wafaa K. Hegazy: Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza 12622, Egypt
Bigad E. Khalil: Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza 12622, Egypt

Clean Technol., 2023, vol. 5, issue 2, 1-15

Abstract: The most abundant organic carbon source on Earth is cellulosic materials. Its main resources are crop straws which are not commonly used and produce environmental pollution. These resources can be a site of biological hydrolysis to primary sugars by cellulase enzymes, in which avicelase is the most efficient enzyme in the cellulase family. This work aimed to clone the avicelase gene, transfer it to E. coli , optimize its expression, saccharify rice straw to its primary sugars, and ferment it to bioethanol. The avicelase gene was cloned from the Bacillus subtilis strain and cloned into two E. coli (i.e., DH5α and Bl21) strains. The optimized avicelase activity was described by testing the effect of different media and growth conditions including different carbon and nitrogen sources, as well as pHs and shaking or static conditions. Avicelase enzyme was extracted and used to saccharify rice straw. The obtained glucose was subjected to fermentation by Saccharomyces cerevisiae F.307 under an aerobic condition growth for the production of bioethanol. The ethanol yield was 5.26% ( v / v ), and the fermentation efficiency was 86%. This study showed the ability to clone one of the cellulolytic genes (i.e., avicelase ) for the valorization of rice straw for producing renewable energy and bioethanol from cellulolytic wastes such as rice straw.

Keywords: rice straw; avicelase gene; cloning; expression optimization; bioethanol (search for similar items in EconPapers)
JEL-codes: Q2 Q3 Q4 Q5 (search for similar items in EconPapers)
Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2571-8797/5/2/23/pdf (application/pdf)
https://www.mdpi.com/2571-8797/5/2/23/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jcltec:v:5:y:2023:i:2:p:23-465:d:1114947

Access Statistics for this article

Clean Technol. is currently edited by Ms. Shary Song

More articles in Clean Technol. from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().