Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon

Natalia Kujawska, Szymon Talbierz, Marcin Dębowski, Joanna Kazimierowicz and Marcin Zieliński
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Natalia Kujawska: InnovaTree Sp. z o.o., 81-451 Gdynia, Poland
Szymon Talbierz: InnovaTree Sp. z o.o., 81-451 Gdynia, Poland
Marcin Dębowski: Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland
Joanna Kazimierowicz: Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, Poland
Marcin Zieliński: Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland

Energies, 2021, vol. 14, issue 10, 1-16

Abstract: Inexpensive carbon sources offering an alternative to glucose are searched for to reduce costs of docosahexaenoic acid production by microalgae. The use of waste glycerol seems substantiated and prospective in this case. The objective of this study was to determine the production yield of heterotrophic microalgae Schizochytrium sp. biomass and the efficiency of docosahexaenoic acid production in various types of cultures with waste glycerol. Cultivation conditions were optimized using the Plackett–Burman method and Response Surface Methodology. The highest technological performance was obtained in the fed-batch culture, where the concentration of Schizochytrium sp. biomass reached 103.44 ± 1.50 g/dm 3 , the lipid concentration in Schizochytrium sp. biomass was at 48.85 ± 0.81 g/dm 3 , and the docosahexaenoic acid concentration at 21.98 ± 0.36 g/dm 3 . The highest docosahexaenoic acid content, accounting for 61.76 ± 3.77% of total fatty acids, was determined in lipid bodies of the Schizochytrium sp. biomass produced in the batch culture, whereas the lowest one, accounting for 44.99 ± 2.12% of total fatty acids, in those of the biomass grown in the fed-batch culture.

Keywords: microalgae; heterotrophic culture; docosahexaenoic acid; glycerol; cultivation methods (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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