Silica/Lignin Carrier as a Factor Increasing the Process Performance and Genetic Diversity of Microbial Communities in Laboratory-Scale Anaerobic Digesters

Agnieszka A. Pilarska, Agnieszka Wolna-Maruwka, Alicja Niewiadomska, Krzysztof Pilarski, Mariusz Adamski, Aleksandra Grzyb, Jarosław Grządziel and Anna Gałązka
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Agnieszka A. Pilarska: Department of Plant-Derived Food Technology, Poznań University of Life Sciences, ul. Wojska Polskiego 31, 60-624 Poznań, Poland
Agnieszka Wolna-Maruwka: Department of General and Environmental Microbiology, Poznań University of Life Sciences, ul. Szydłowska 50, 60-656 Poznań, Poland
Alicja Niewiadomska: Department of General and Environmental Microbiology, Poznań University of Life Sciences, ul. Szydłowska 50, 60-656 Poznań, Poland
Krzysztof Pilarski: Department of Biosystems Engineering, Poznań University of Life Sciences, ul. Wojska Polskiego 50, 60-627 Poznań, Poland
Mariusz Adamski: Department of Biosystems Engineering, Poznań University of Life Sciences, ul. Wojska Polskiego 50, 60-627 Poznań, Poland
Aleksandra Grzyb: Department of General and Environmental Microbiology, Poznań University of Life Sciences, ul. Szydłowska 50, 60-656 Poznań, Poland
Jarosław Grządziel: Department of Agriculture Microbiology, Institute of Soil Science and Plant Cultivation–State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland
Anna Gałązka: Department of Agriculture Microbiology, Institute of Soil Science and Plant Cultivation–State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland

Energies, 2021, vol. 14, issue 15, 1-22

Abstract: The article aims to present results of research on anaerobic digestion (AD) of waste wafers (WF-control) and co-substrate system–waste wafers and cheese (WFC-control), combined with digested sewage sludge, as inoculum. The purpose of this paper is to confirm the outcome of adding silica/lignin (S/L; 4:1) material, as a microbial carrier, on the process performance and genetic diversity of microbial communities. The experiment was conducted in a laboratory under mesophilic conditions, in a periodical operation mode of bioreactors. Selected physicochemical parameters of the tested carrier, along with the microstructure and thermal stability, were determined. Substrates, batches and fermenting slurries were subjected to standard parameter analysis. As part of the conducted analysis, samples of fermented food were also tested for total bacterial count, dehydrogenase activity. Additionally, DNA extraction and next-generation sequencing (NGS) were carried out. As a result of the conducted study, an increase in the volume of produced biogas was recorded for samples fermented with S/L carrier: in the case of WF + S/L by 18.18% to a cumulative biogas yield of 833.35 m 3 Mg ?1 VS, and in the case of WFC + S/L by 17.49% to a yield of 950.64 m 3 Mg ?1 VS. The largest total bacterial count, during the process of dehydrogenase activity, was maintained in the WFC + S/L system. The largest bacterial biodiversity was recorded in samples fermented with the addition of cheese, both in the case of the control variant and in the variant when the carrier was used. In contrast, three phyla of bacteria Firmicutes , Proteobacteria and Actinobacteria predominated in all experimental facilities.

Keywords: silica/lignin carrier; organic waste; anaerobic digestion; microbial genetic diversity; process performance (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

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