Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Sunan Nuanpeng, Sudarat Thanonkeo, Mamoru Yamada and Pornthap Thanonkeo
Additional contact information
Sunan Nuanpeng: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
Sudarat Thanonkeo: Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
Mamoru Yamada: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
Pornthap Thanonkeo: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2016, vol. 9, issue 4, 1-20

Abstract: Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of thermotolerant yeasts capable of growth and ethanol production at high temperatures were successfully isolated. Among these isolates, the newly isolated thermotolerant yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great potential for ethanol production from sweet sorghum juice (SSJ) at high temperatures. The maximum ethanol concentrations produced by this newly isolated thermotolerant yeast at 37 °C and 40 °C under the optimum cultural condition were 106.82 g·L ?1 and 85.01 g·L ?1 , respectively, which are greater than values reported in the literatures. It should be noted from this study with SSJ at a sugar concentration of 250 g·L ?1 and an initial pH of 5.5 without nitrogen supplementation can be used directly as substrate for ethanol production at high temperatures by thermotolerant yeast S. cerevisiae DBKKU Y-53. Gene expression analysis using real-time RT-PCR clearly indicated that growth and ethanol fermentation activities of the thermotolerant yeast S. cerevisiae DBKKU Y-53 at a high temperature (40 °C) were not only restricted to the expression of genes involved in the heat-shock response, but also to those genes involved in ATP production, trehalose and glycogen metabolism, and protein degradation processes were also involved.

Keywords: high temperature fermentation; real-time RT-PCR; Saccharomyces cerevisiae; sweet sorghum; thermotolerant yeast; gene expression (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2016
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.mdpi.com/1996-1073/9/4/253/pdf (application/pdf)
https://www.mdpi.com/1996-1073/9/4/253/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jeners:v:9:y:2016:i:4:p:253-:d:66937

Access Statistics for this article

Energies is currently edited by Ms. Agatha Cao

More articles in Energies from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().