Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5?-Fluorodeoxy Adenosine

Ningning Li, Bingjing Hu, Anming Wang, Huimin Li, Youcheng Yin, Tianyu Mao and Tian Xie
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Ningning Li: College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
Bingjing Hu: College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
Anming Wang: College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
Huimin Li: College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
Youcheng Yin: Holistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University, Hangzhou 311121, China
Tianyu Mao: College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
Tian Xie: Holistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University, Hangzhou 311121, China

Sustainability, 2020, vol. 12, issue 1, 1-15

Abstract: To develop an environmentally friendly biocatalyst for the efficient synthesis of organofluorine compounds, we prepared the enzyme@fluoridated hydroxyapatite nanoflowers (FHAp-NFs) using fluorinase expressed in Escherichia coli Rosetta (DE3) as the biomineralization framework. The obtained fluorinase@FHAp-NFs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and FT-IR spectrum and used in the enzymatic synthesis of 5′-fluorodeoxy adenosin with S-adenosyl-L-methionine and fluoride as substrate. At an optimum pH of 7.5, fluorinase confined in the hybrid nanoflowers presents an approximately 2-fold higher synthetic activity than free fluorinase. Additionally, after heating at 30 °C for 8 h, the FHAp-NFs retained approximately 80.0% of the initial activity. However, free enzyme could remain only 48.2% of its initial activity. The results indicate that the fluoride and hybrid nanoflowers efficiently enhance the catalytic activity and thermal stability of fluorinase in the synthesis of 5′-fluorodeoxy adenosine, which gives a green method for producing the fluorinated organic compounds.

Keywords: organofluorine compound; fluorinase; 5?-fluorodeoxy adenosine; biomineralization; immobilization; fluoridated hydroxyapatite nanoflowers (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
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