Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

M. Asimuddin, Mohammed Rafi Shaik, Neeshat Fathima, M. Shaistha Afreen, Syed Farooq Adil, Mohammed Rafiq H. Siddiqui, Kaiser Jamil and Mujeeb Khan
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M. Asimuddin: Center for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad 500003, Telangana, India
Mohammed Rafi Shaik: Department of Chemistry, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia
Neeshat Fathima: Center for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad 500003, Telangana, India
M. Shaistha Afreen: Center for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad 500003, Telangana, India
Syed Farooq Adil: Department of Chemistry, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia
Mohammed Rafiq H. Siddiqui: Department of Chemistry, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia
Kaiser Jamil: Center for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad 500003, Telangana, India
Mujeeb Khan: Department of Chemistry, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia

Sustainability, 2020, vol. 12, issue 4, 1-14

Abstract: Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl 3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa, and Bacillus subtilis . The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

Keywords: Ziziphus mauritiana; green synthesis; plant extracts; silver NPs; biological activity (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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