Influence of Light Intensity and Photoperiod on the Photoautotrophic Growth and Lipid Content of the Microalgae Verrucodesmus verrucosus in a Photobioreactor

Laura Vélez-Landa, Héctor Ricardo Hernández- De León, Yolanda Del Carmen Pérez-Luna, Sabino Velázquez-Trujillo, Joel Moreira-Acosta, Roberto Berrones-Hernández and Yazmin Sánchez-Roque
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Laura Vélez-Landa: Research Laboratory, Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana KM. 1080, Tuxtla Gutiérrez C.P. 29050, Mexico
Héctor Ricardo Hernández- De León: Research Laboratory, Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana KM. 1080, Tuxtla Gutiérrez C.P. 29050, Mexico
Yolanda Del Carmen Pérez-Luna: Research Laboratory, Universidad Politécnica de Chiapas, Carretera Tuxtla-Villaflores KM. 1+500, Las Brisas, Suchiapa C.P. 29150, Mexico
Sabino Velázquez-Trujillo: Research Laboratory, Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana KM. 1080, Tuxtla Gutiérrez C.P. 29050, Mexico
Joel Moreira-Acosta: Research Laboratory, Universidad del Valle de México, Tuxtla Gutiérrez, Chiapas , C.P. 29056, México
Roberto Berrones-Hernández: Research Laboratory, Universidad Politécnica de Chiapas, Carretera Tuxtla-Villaflores KM. 1+500, Las Brisas, Suchiapa C.P. 29150, Mexico
Yazmin Sánchez-Roque: Research Laboratory, Universidad Politécnica de Chiapas, Carretera Tuxtla-Villaflores KM. 1+500, Las Brisas, Suchiapa C.P. 29150, Mexico

Sustainability, 2021, vol. 13, issue 12, 1-11

Abstract: Microalgal biomass has the capacity to accumulate relatively large quantities of triacylglycerides (TAG) for the conversion of methyl esters of fatty acids (FAME) which has made microalgae a desirable alternative for the production of biofuels. In the present work Verrucodesmus verrucosus was evaluated under autotrophic growth conditions as a suitable source of oil for biodiesel production. For this purpose BG11 media were evaluated in three different light:dark photoperiods (L:D; 16:08; 12:12; 24:0) and light intensities (1000, 2000 and 3000 Lux) in a photobioreactor with a capacity of three liters; the evaluation of the microalgal biomass was carried out through the cell count with the use of the Neubauer chamber followed by the evaluation of the kinetic growth parameters. So, the lipid accumulation was determined through the lipid extraction with a Soxhlet system. Finally, the fatty acid profile of the total pooled lipids was determined using gas chromatography-mass spectroscopy (GC-MS). The results demonstrate that the best conditions are a photoperiod of 12 light hours and 12 dark hours with BG11 medium in a 3 L tubular photobioreactor with 0.3% CO 2 , 25 °C and 2000 Lux, allowing a lipid accumulation of 50.42%. Palmitic acid is identified as the most abundant fatty acid at 44.90%.

Keywords: photobioreactor; light intensity; microalgal biomass; Verrucodesmus verrucosus (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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