On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste via Yeast Co-Cultures

Mohamed Hashem, Saad A. Alamri, Tahani A. Y. Asseri, Yasser S. Mostafa, Gerasimos Lyberatos and Ioanna Ntaikou
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Mohamed Hashem: Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
Saad A. Alamri: Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
Tahani A. Y. Asseri: Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
Yasser S. Mostafa: Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
Gerasimos Lyberatos: Zografou Campus, School of Chemical Engineering, National Technical University of Athens, GR 15780 Athens, Greece
Ioanna Ntaikou: Zografou Campus, School of Chemical Engineering, National Technical University of Athens, GR 15780 Athens, Greece

Sustainability, 2021, vol. 13, issue 4, 1-13

Abstract: The present study aims to assess the impact of the type of yeast consortium used during bioethanol production from starchy biowastes and to determine the optimal fermentation conditions for enhanced bioethanol production. Three different yeast strains, Saccharomyces cerevisiae , Pichia barkeri, and Candida intermedia were used in mono- and co-cultures with pretreated waste-rice as substrate. The optimization of fermentation conditions i.e., fermentation time, temperature, pH, and inoculum size, was investigated in small-scale batch cultures and subsequently, the optimal conditions were applied for scaling-up and validation of the process in a 7-L fermenter. It was shown that co-culturing of yeasts either in couples or triples significantly enhanced the fermentation efficiency of the process, with ethanol yield reaching 167.80 ± 0.49 g/kg of biowaste during experiments in the fermenter.

Keywords: bioethanol; starchy wastes; hydrolysate; co-cultures; scale-up (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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