Sustainable Biodiesel Production from a New Oleaginous Fungus, Aspergillus carneus Strain OQ275240: Biomass and Lipid Production Optimization Using Box–Behnken Design

Amany G. Ibrahim (), Alaa Baazeem, Mayasar I. Al-Zaban, Mustafa A. Fawzy (), Sedky H. A. Hassan and Mostafa Koutb
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Amany G. Ibrahim: Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11566, Egypt
Alaa Baazeem: Biology Department, Faculty of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Mayasar I. Al-Zaban: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Mustafa A. Fawzy: Biology Department, Faculty of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Sedky H. A. Hassan: Department of Biology, College of Science, Sultan Qaboos University, Muscat 123, Oman
Mostafa Koutb: Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt

Sustainability, 2023, vol. 15, issue 8, 1-17

Abstract: Due to their low cost and ability to synthesize lipids for sustainable biodiesel production, oleaginous fungus has recently gained more prominence than other microorganisms. The new oleaginous fungus Aspergillus carneus OQ275240’s dry biomass, lipid content, and lipid yield were all optimized in this work, using the response surface methodology-based Box–Behnken design. Analysis of variance (ANOVA) was also used to examine the experimental data, and multiple regression analysis was used to fit the data to a second-order polynomial equation. Three independent variables, such as the concentration of yeast, glucose, and phosphorus, were examined for their mutual impacts. Maximum dry biomass (0.024 g/50 mL), lipid content (36.20%), and lipid yield (8.70 mg/50 mL) were achieved at optimal concentrations of 2.68 g/L of yeast, 20.82 g/L of glucose, and 0.10 g/L of phosphorus, respectively, showing that the actual data and predictions of the models were in good agreement. A. carneus OQ275240 has a favorable fatty acid profile that can be used to successfully create biodiesel, as shown by the presence of palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1) in its fatty acid methyl esters (FAMEs) profile. Furthermore, the qualities of the biodiesel were investigated, and it was found that they fell within the parameters established by the international specifications EN 14214 (Europe) and ASTM D6751-08 (United States). These findings point to the newly evaluated filamentous fungal strain as a potential feedstock for the production of high-quality biodiesel.

Keywords: Aspergillus carneus; biodiesel; Box–Behnken design; lipids; oleaginous fungi; optimization (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2023
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