Reprogramming microbial populations using a programmed lysis system to improve chemical production

Wenwen Diao, Liang Guo, Qiang Ding, Cong Gao, Guipeng Hu, Xiulai Chen, Yang Li, Linpei Zhang, Wei Chen, Jian Chen and Liming Liu ()
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Wenwen Diao: State Key Laboratory of Food Science and Technology, Jiangnan University
Liang Guo: State Key Laboratory of Food Science and Technology, Jiangnan University
Qiang Ding: State Key Laboratory of Food Science and Technology, Jiangnan University
Cong Gao: State Key Laboratory of Food Science and Technology, Jiangnan University
Guipeng Hu: State Key Laboratory of Food Science and Technology, Jiangnan University
Xiulai Chen: State Key Laboratory of Food Science and Technology, Jiangnan University
Yang Li: State Key Laboratory of Food Science and Technology, Jiangnan University
Linpei Zhang: School of Biotechnology, Jiangnan University
Wei Chen: State Key Laboratory of Food Science and Technology, Jiangnan University
Jian Chen: School of Biotechnology, Jiangnan University
Liming Liu: State Key Laboratory of Food Science and Technology, Jiangnan University

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Microbial populations are a promising model for achieving microbial cooperation to produce valuable chemicals. However, regulating the phenotypic structure of microbial populations remains challenging. In this study, a programmed lysis system (PLS) is developed to reprogram microbial cooperation to enhance chemical production. First, a colicin M -based lysis unit is constructed to lyse Escherichia coli. Then, a programmed switch, based on proteases, is designed to regulate the effective lysis unit time. Next, a PLS is constructed for chemical production by combining the lysis unit with a programmed switch. As a result, poly (lactate-co-3-hydroxybutyrate) production is switched from PLH synthesis to PLH release, and the content of free PLH is increased by 283%. Furthermore, butyrate production with E. coli consortia is switched from E. coli BUT003 to E. coli BUT004, thereby increasing butyrate production to 41.61 g/L. These results indicate the applicability of engineered microbial populations for improving the metabolic division of labor to increase the efficiency of microbial cell factories.

Date: 2021
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DOI: 10.1038/s41467-021-27226-3

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