110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics
Miroslav Stibůrek,
Petra Ondráčková,
Tereza Tučková,
Sergey Turtaev,
Martin Šiler,
Tomáš Pikálek,
Petr Jákl,
André Gomes,
Jana Krejčí,
Petra Kolbábková,
Hana Uhlířová (huhlirova@isibrno.cz) and
Tomáš Čižmár (cizmart@isibrno.cz)
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Miroslav Stibůrek: Institute of Scientific Instruments of the Czech Academy of Sciences
Petra Ondráčková: Institute of Scientific Instruments of the Czech Academy of Sciences
Tereza Tučková: Institute of Scientific Instruments of the Czech Academy of Sciences
Sergey Turtaev: Leibniz Institute of Photonic Technology
Martin Šiler: Institute of Scientific Instruments of the Czech Academy of Sciences
Tomáš Pikálek: Institute of Scientific Instruments of the Czech Academy of Sciences
Petr Jákl: Institute of Scientific Instruments of the Czech Academy of Sciences
André Gomes: Leibniz Institute of Photonic Technology
Jana Krejčí: Institute of Biophysics of the Czech Academy of Sciences
Petra Kolbábková: Institute of Scientific Instruments of the Czech Academy of Sciences
Hana Uhlířová: Institute of Scientific Instruments of the Czech Academy of Sciences
Tomáš Čižmár: Institute of Scientific Instruments of the Czech Academy of Sciences
Nature Communications, 2023, vol. 14, issue 1, 1-9
Abstract:
Abstract Light-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces imaging contrast and resolution, making it difficult to reach structures at greater depths even with the use of multiphoton techniques. To reach deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses and enable a variety of modalities in head-fixed and freely moving animals. A recently proposed alternative is the use of holographic control of light transport through multimode optical fibres promising much less traumatic application and superior imaging performance. We present a 110 μm thin laser-scanning endo-microscope based on this prospect, enabling in-vivo volumetric imaging throughout the whole depth of the mouse brain. The instrument is equipped with multi-wavelength detection and three-dimensional random access options, and it performs at lateral resolution below 1 μm. We showcase various modes of its application through the observations of fluorescently labelled neurones, their processes and blood vessels. Finally, we demonstrate how to exploit the instrument to monitor calcium signalling of neurones and to measure blood flow velocity in individual vessels at high speeds.
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36889-z
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DOI: 10.1038/s41467-023-36889-z
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