Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages

Mark Chernyshev, Mrunal Sakharkar, Ruth I. Connor, Haley L. Dugan, Daniel J. Sheward, C. G. Rappazzo, Aron Stålmarck, Mattias N. E. Forsell, Peter F. Wright, Martin Corcoran, Ben Murrell, Laura M. Walker () and Gunilla B. Karlsson Hedestam ()
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Mark Chernyshev: Tumor and Cell Biology, Karolinska Institutet
Mrunal Sakharkar: Adimab LLC
Ruth I. Connor: Dartmouth-Hitchcock Medical Center
Haley L. Dugan: Adimab LLC
Daniel J. Sheward: Tumor and Cell Biology, Karolinska Institutet
C. G. Rappazzo: Adimab LLC
Aron Stålmarck: Tumor and Cell Biology, Karolinska Institutet
Mattias N. E. Forsell: Umeå University
Peter F. Wright: Dartmouth-Hitchcock Medical Center
Martin Corcoran: Tumor and Cell Biology, Karolinska Institutet
Ben Murrell: Tumor and Cell Biology, Karolinska Institutet
Laura M. Walker: Adimab LLC
Gunilla B. Karlsson Hedestam: Tumor and Cell Biology, Karolinska Institutet

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract Vaccination of SARS-CoV-2 convalescent individuals generates broad and potent antibody responses. Here, we isolate 459 spike-specific monoclonal antibodies (mAbs) from two individuals who were infected with the index variant of SARS-CoV-2 and later boosted with mRNA-1273. We characterize mAb genetic features by sequence assignments to the donors’ personal immunoglobulin genotypes and assess antibody neutralizing activities against index SARS-CoV-2, Beta, Delta, and Omicron variants. The mAbs used a broad range of immunoglobulin heavy chain (IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors. IGH repertoire sequencing and B cell lineage tracing at longitudinal time points reveals extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection until vaccination five months later. These results demonstrate that highly polyclonal repertoires of affinity-matured memory B cells are efficiently recalled by vaccination, providing a basis for the potent antibody responses observed in convalescent persons following vaccination.

Date: 2023
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DOI: 10.1038/s41467-023-37972-1

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