EconPapers    
Economics at your fingertips  
 

SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle

Liv Zimmermann, Xiaohan Zhao, Jana Makroczyova, Moritz Wachsmuth-Melm, Vibhu Prasad, Zach Hensel, Ralf Bartenschlager and Petr Chlanda (petr.chlanda@bioquant.uni-heidelberg.de)
Additional contact information
Liv Zimmermann: Virology, Heidelberg University
Xiaohan Zhao: Virology, Heidelberg University
Jana Makroczyova: Virology, Heidelberg University
Moritz Wachsmuth-Melm: Virology, Heidelberg University
Vibhu Prasad: Heidelberg University
Zach Hensel: Universidade NOVA de Lisboa
Ralf Bartenschlager: Heidelberg University
Petr Chlanda: Virology, Heidelberg University

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract Coronavirus replication is associated with the remodeling of cellular membranes, resulting in the formation of double-membrane vesicles (DMVs). A DMV-spanning pore was identified as a putative portal for viral RNA. However, the exact components and the structure of the SARS-CoV-2 DMV pore remain to be determined. Here, we investigate the structure of the DMV pore by in situ cryo-electron tomography combined with subtomogram averaging. We identify non-structural protein (nsp) 3 and 4 as minimal components required for the formation of a DMV-spanning pore, which is dependent on nsp3-4 proteolytic cleavage. In addition, we show that Mac2-Mac3-DPUP-Ubl2 domains are critical for nsp3 oligomerization and crown integrity which influences membrane curvature required for biogenesis of DMVs. Altogether, SARS-CoV-2 nsp3-4 have a dual role by driving the biogenesis of replication organelles and assembly of DMV-spanning pores which we propose here to term replicopores.

Date: 2023
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-023-43666-5 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43666-5

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-43666-5

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla (sonal.shukla@springer.com) and Springer Nature Abstracting and Indexing (indexing@springernature.com).

 
Page updated 2024-12-28
Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43666-5