Phosphorylation of plasma membrane H+-ATPase Thr881 participates in light-induced stomatal opening

Yuki Hayashi, Kohei Fukatsu, Koji Takahashi, Satoru N. Kinoshita, Kyohei Kato, Taku Sakakibara, Keiko Kuwata and Toshinori Kinoshita ()
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Yuki Hayashi: Nagoya University
Kohei Fukatsu: Nagoya University
Koji Takahashi: Nagoya University
Satoru N. Kinoshita: Nagoya University
Kyohei Kato: Nagoya University
Taku Sakakibara: Nagoya University
Keiko Kuwata: Nagoya University
Toshinori Kinoshita: Nagoya University

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Plasma membrane (PM) H+-ATPase is crucial for light-induced stomatal opening and phosphorylation of a penultimate residue, Thr948 (pen-Thr, numbering according to Arabidopsis AHA1) is required for enzyme activation. In this study, a comprehensive phosphoproteomic analysis using guard cell protoplasts from Vicia faba shows that both red and blue light increase the phosphorylation of Thr881, of PM H+-ATPase. Light-induced stomatal opening and the blue light-induced increase in stomatal conductance are reduced in transgenic Arabidopsis plants expressing mutant AHA1-T881A in aha1–9, whereas the blue light-induced phosphorylation of pen-Thr is unaffected. Auxin and photosynthetically active radiation induce the phosphorylation of both Thr881 and pen-Thr in etiolated seedlings and leaves, respectively. The dephosphorylation of phosphorylated Thr881 and pen-Thr are mediated by type 2 C protein phosphatase clade D isoforms. Taken together, Thr881 phosphorylation, in addition of the pen-Thr phosphorylation, are important for PM H+-ATPase function during physiological responses, such as light-induced stomatal opening in Arabidopsis thaliana.

Date: 2024
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DOI: 10.1038/s41467-024-45248-5

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