Synapsin 2a tetramerisation selectively controls the presynaptic nanoscale organisation of reserve synaptic vesicles

Shanley F. Longfield, Rachel S. Gormal, Matis Feller, Pierre Parutto, Jürgen Reingruber, Tristan P. Wallis, Merja Joensuu, George J. Augustine, Ramón Martínez-Mármol, David Holcman and Frédéric A. Meunier ()
Additional contact information
Shanley F. Longfield: The University of Queensland
Rachel S. Gormal: The University of Queensland
Matis Feller: IBENS, Ecole Normale Superieure
Pierre Parutto: IBENS, Ecole Normale Superieure
Jürgen Reingruber: IBENS, Ecole Normale Superieure
Tristan P. Wallis: The University of Queensland
Merja Joensuu: The University of Queensland
George J. Augustine: Temasek Lifesciences Laboratory
Ramón Martínez-Mármol: The University of Queensland
David Holcman: IBENS, Ecole Normale Superieure
Frédéric A. Meunier: The University of Queensland

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract Neurotransmitter release relies on the regulated fusion of synaptic vesicles (SVs) that are tightly packed within the presynaptic bouton of neurons. The mechanism by which SVs are clustered at the presynapse, while preserving their ability to dynamically recycle to support neuronal communication, remains unknown. Synapsin 2a (Syn2a) tetramerization has been suggested as a potential clustering mechanism. Here, we used Dual-pulse sub-diffractional Tracking of Internalised Molecules (DsdTIM) to simultaneously track single SVs from the recycling and the reserve pools, in live hippocampal neurons. The reserve pool displays a lower presynaptic mobility compared to the recycling pool and is also present in the axons. Triple knockout of Synapsin 1-3 genes (SynTKO) increased the mobility of reserve pool SVs. Re-expression of wild-type Syn2a (Syn2aWT), but not the tetramerization-deficient mutant K337Q (Syn2aK337Q), fully rescued these effects. Single-particle tracking revealed that Syn2aK337QmEos3.1 exhibited altered activity-dependent presynaptic translocation and nanoclustering. Therefore, Syn2a tetramerization controls its own presynaptic nanoclustering and thereby contributes to the dynamic immobilisation of the SV reserve pool.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-46256-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46256-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-46256-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().