ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA

Xinlei Wei, Xue Yang, Congcong Hu, Qiangzi Li, Qianqian Liu, Yue Wu, Leipeng Xie, Xiao Ning, Fei Li, Tao Cai, Zhiguang Zhu, Yi-Heng P. Job Zhang, Yanfei Zhang, Xuejun Chen and Chun You ()
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Xinlei Wei: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Xue Yang: Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Congcong Hu: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Qiangzi Li: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Qianqian Liu: Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Yue Wu: Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Leipeng Xie: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Xiao Ning: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Fei Li: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Tao Cai: Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Zhiguang Zhu: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Yi-Heng P. Job Zhang: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Yanfei Zhang: Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Xuejun Chen: Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
Chun You: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate. Stoichiometric analysis indicates this ivSEB can self-maintain NADP+/NADPH balance and achieve a theoretical molar yield of 133.3%. Leveraging simple one-pot reactions, our ivSEBs achieved a near-theoretical molar yield of 125.5%, the highest PHB titer (208.3 mM, approximately 17.9 g/L) and the fastest PHB production rate (9.4 mM/h, approximately 0.8 g/L/h) among all the reported ivSEBs to date, and demonstrated easy scalability. This study unveils the promising potential of ivBT for the industrial-scale production of PHB and other acetyl-CoA-derived chemicals from starch.

Date: 2024
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DOI: 10.1038/s41467-024-46871-y

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