Multicore fiber optic imaging reveals that astrocyte calcium activity in the mouse cerebral cortex is modulated by internal motivational state

Yung-Tian A. Gau (), Eric T. Hsu, Richard J. Cha, Rebecca W. Pak, Loren L. Looger, Jin U. Kang () and Dwight E. Bergles ()
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Yung-Tian A. Gau: Johns Hopkins University
Eric T. Hsu: Johns Hopkins University
Richard J. Cha: Johns Hopkins University
Rebecca W. Pak: Johns Hopkins University
Loren L. Looger: University of California San Diego
Jin U. Kang: Johns Hopkins University
Dwight E. Bergles: Johns Hopkins University

Nature Communications, 2024, vol. 15, issue 1, 1-21

Abstract: Abstract Astrocytes are a direct target of neuromodulators and can influence neuronal activity on broad spatial and temporal scales in response to a rise in cytosolic calcium. However, our knowledge about how astrocytes are recruited during different animal behaviors remains limited. To measure astrocyte activity calcium in vivo during normative behaviors, we utilize a high-resolution, long working distance multicore fiber optic imaging system that allows visualization of individual astrocyte calcium transients in the cerebral cortex of freely moving mice. We define the spatiotemporal dynamics of astrocyte calcium changes during diverse behaviors, ranging from sleep-wake cycles to the exploration of novel objects, showing that their activity is more variable and less synchronous than apparent in head-immobilized imaging conditions. In accordance with their molecular diversity, individual astrocytes often exhibit distinct thresholds and activity patterns during explorative behaviors, allowing temporal encoding across the astrocyte network. Astrocyte calcium events were induced by noradrenergic and cholinergic systems and modulated by internal state. The distinct activity patterns exhibited by astrocytes provides a means to vary their neuromodulatory influence in different behavioral contexts and internal states.

Date: 2024
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DOI: 10.1038/s41467-024-47345-x

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