Diverse and abundant phages exploit conjugative plasmids

Natalia Quinones-Olvera, Siân V. Owen (), Lucy M. McCully, Maximillian G. Marin, Eleanor A. Rand, Alice C. Fan, Oluremi J. Martins Dosumu, Kay Paul, Cleotilde E. Sanchez Castaño, Rachel Petherbridge, Jillian S. Paull and Michael Baym ()
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Natalia Quinones-Olvera: Harvard Medical School
Siân V. Owen: Harvard Medical School
Lucy M. McCully: Harvard Medical School
Maximillian G. Marin: Harvard Medical School
Eleanor A. Rand: Harvard Medical School
Alice C. Fan: Harvard Medical School
Oluremi J. Martins Dosumu: Harvard Medical School
Kay Paul: Harvard Medical School
Cleotilde E. Sanchez Castaño: Harvard Medical School
Rachel Petherbridge: Harvard Medical School
Jillian S. Paull: Harvard Medical School
Michael Baym: Harvard Medical School

Nature Communications, 2024, vol. 15, issue 1, 1-16

Abstract: Abstract Phages exert profound evolutionary pressure on bacteria by interacting with receptors on the cell surface to initiate infection. While the majority of phages use chromosomally encoded cell surface structures as receptors, plasmid-dependent phages exploit plasmid-encoded conjugation proteins, making their host range dependent on horizontal transfer of the plasmid. Despite their unique biology and biotechnological significance, only a small number of plasmid-dependent phages have been characterized. Here we systematically search for new plasmid-dependent phages targeting IncP and IncF plasmids using a targeted discovery platform, and find that they are common and abundant in wastewater, and largely unexplored in terms of their genetic diversity. Plasmid-dependent phages are enriched in non-canonical types of phages, and all but one of the 65 phages we isolated were non-tailed, and members of the lipid-containing tectiviruses, ssDNA filamentous phages or ssRNA phages. We show that plasmid-dependent tectiviruses exhibit profound differences in their host range which is associated with variation in the phage holin protein. Despite their relatively high abundance in wastewater, plasmid-dependent tectiviruses are missed by metaviromic analyses, underscoring the continued importance of culture-based phage discovery. Finally, we identify a tailed phage dependent on the IncF plasmid, and find related structural genes in phages that use the orthogonal type 4 pilus as a receptor, highlighting the evolutionarily promiscuous use of these distinct contractile structures by multiple groups of phages. Taken together, these results indicate plasmid-dependent phages play an under-appreciated evolutionary role in constraining horizontal gene transfer via conjugative plasmids.

Date: 2024
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DOI: 10.1038/s41467-024-47416-z

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