A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

Miaomiao Li (), Huaizhi Zhang, Huixin Xiao, Keyu Zhu, Wenqi Shi, Dong Zhang, Yong Wang, Lijun Yang, Qiuhong Wu, Jingzhong Xie, Yongxing Chen, Dan Qiu, Guanghao Guo, Ping Lu, Beibei Li, Lei Dong, Wenling Li, Xuejia Cui, Lingchuan Li, Xiubin Tian, Chengguo Yuan, Yiwen Li, Dazhao Yu, Eviatar Nevo, Tzion Fahima, Hongjie Li, Lingli Dong (), Yusheng Zhao () and Zhiyong Liu ()
Additional contact information
Miaomiao Li: Chinese Academy of Sciences
Huaizhi Zhang: Chinese Academy of Sciences
Huixin Xiao: Chinese Academy of Sciences
Keyu Zhu: Chinese Academy of Sciences
Wenqi Shi: Hubei Academy of Agricultural Sciences
Dong Zhang: Beijing PlantTech Biotechnology Co., Ltd.
Yong Wang: Chinese Academy of Agricultural Sciences
Lijun Yang: Hubei Academy of Agricultural Sciences
Qiuhong Wu: Chinese Academy of Sciences
Jingzhong Xie: Chinese Academy of Sciences
Yongxing Chen: Chinese Academy of Sciences
Dan Qiu: Chinese Academy of Sciences
Guanghao Guo: Chinese Academy of Sciences
Ping Lu: Chinese Academy of Sciences
Beibei Li: Chinese Academy of Sciences
Lei Dong: Chinese Academy of Sciences
Wenling Li: Chinese Academy of Sciences
Xuejia Cui: Chinese Academy of Sciences
Lingchuan Li: Chinese Academy of Sciences
Xiubin Tian: Chinese Academy of Sciences
Chengguo Yuan: Hebei Gaoyi Seeds Farm
Yiwen Li: Chinese Academy of Sciences
Dazhao Yu: Hubei Academy of Agricultural Sciences
Eviatar Nevo: University of Haifa
Tzion Fahima: University of Haifa
Hongjie Li: Chinese Academy of Agricultural Sciences
Lingli Dong: Chinese Academy of Sciences
Yusheng Zhao: Chinese Academy of Sciences
Zhiyong Liu: Chinese Academy of Sciences

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.

Date: 2024
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DOI: 10.1038/s41467-024-47497-w

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