Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions

Saori Yoshimura, Ryuki Shimada, Koji Kikuchi, Soichiro Kawagoe, Hironori Abe, Sakie Iisaka, Sayoko Fujimura, Kei-ichiro Yasunaga, Shingo Usuki, Naoki Tani, Takashi Ohba, Eiji Kondoh, Tomohide Saio, Kimi Araki and Kei-ichiro Ishiguro ()
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Saori Yoshimura: Kumamoto University
Ryuki Shimada: Kumamoto University
Koji Kikuchi: Kumamoto University
Soichiro Kawagoe: Tokushima University
Hironori Abe: Kumamoto University
Sakie Iisaka: Kumamoto University
Sayoko Fujimura: Kumamoto University
Kei-ichiro Yasunaga: Kumamoto University
Shingo Usuki: Kumamoto University
Naoki Tani: Kumamoto University
Takashi Ohba: Kumamoto University
Eiji Kondoh: Kumamoto University
Tomohide Saio: Tokushima University
Kimi Araki: Kumamoto University
Kei-ichiro Ishiguro: Kumamoto University

Nature Communications, 2024, vol. 15, issue 1, 1-22

Abstract: Abstract Meiotic prophase progression is differently regulated in males and females. In males, pachytene transition during meiotic prophase is accompanied by robust alteration in gene expression. However, how gene expression is regulated differently to ensure meiotic prophase completion in males remains elusive. Herein, we identify HSF5 as a male germ cell-specific heat shock transcription factor (HSF) for meiotic prophase progression. Genetic analyzes and single-cell RNA-sequencing demonstrate that HSF5 is essential for progression beyond the pachytene stage under non-stress conditions rather than heat stress. Chromatin binding analysis in vivo and DNA-binding assays in vitro suggest that HSF5 binds to promoters in a subset of genes associated with chromatin organization. HSF5 recognizes a DNA motif different from typical heat shock elements recognized by other canonical HSFs. This study suggests that HSF5 is an atypical HSF that is required for the gene expression program for pachytene transition during meiotic prophase in males.

Date: 2024
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DOI: 10.1038/s41467-024-47601-0

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